Table 1.
Bacterial strains tested by PCR-hly
| Bacteriuma | Referenceb | Result of PCR-hlyc |
|---|---|---|
| Listeriae | ||
| L. monocytogenes EGD-e 1/2a (LLO) | Sequenced strain EGD-e (20) | Positive |
| L. monocytogenes LO28 1/2c (LLO) | Sequenced strain L028 | Positive |
| L. monocytogenes 1/2a (LLO) | Serovar reference strain CLIP 74902 | Positive |
| L. monocytogenes 1/2b (LLO) | Serovar reference strain CLIP 74903 | Positive |
| L. monocytogenes 1/2c (LLO) | Serovar reference strain CLIP 74904 | Positive |
| L. monocytogenes 4b (LLO) | Serovar reference strain CLIP 74910 | Positive |
| L. monocytogenes 4b (LLO) | Clinical strain | Positive |
| L. ivanovii 5 (IVO) | Reference strain CIP 12229, CIP 7842 | Negative |
| L. seeligerii 1/2b (LSO) | Reference strain CIP 73021 | Negative |
| L. innocua 6a | Sequenced strain, CIP 11262 (20) | Negative |
| Gram-positive bacteria secreting a pore-forming cytolysin | ||
| Streptococcus pneumoniae (PLY) | Clinical strain | Negative |
| S. pyogenes (SLO) | Clinical strain | Negative |
| S. dysgalactiae subsp. dysgalactiae (SLO) | Clinical strain | Negative |
| S. dysgalactiae subsp. equisimilis (SLO) | Clinical strain | Negative |
| Clostridium perfringens (PFO) | Clinical strain | Negative |
| Bacillus cereus (CLY) | Clinical strain | Negative |
| Other bacterial strains frequently associated with community or nosocomial CNS infections | ||
| Neisseria meningitidis | Sequenced strain Z2491 | Negative |
| Streptococcus agalactiae (group B) | Sequenced strain Nem316 | Negative |
| Haemophilus influenzae (group B) | Clinical strain | Negative |
| Escherichia coli antigen K1 | Clinical strain | Negative |
| Staphylococcus aureus | Clinical strain | Negative |
| Staphylococcus epidermidis (Ica negative)d | CIP 68.21 | Negative |
| Staphylococcus epidermidis (Ica positive)d | CIP 105.777 | Negative |
| Pseudomonas aeruginosa | Clinical strain | Negative |
| Mycobacterium tuberculosis | Clinical strain | Negative |
a
When the bacterium secreted a thiol-activated pore-forming cytolysin, its abbreviation is indicated in parentheses: CLY, cereolysin; IVO, ivanolysin O; LLO, listeriolysin O; LSO, seeligerilysin O; PFO, perfringolysin; PLY, pneumolysin; SLO, streptolysin O.
b
CIP, Collection of Institut Pasteur; CLIP, Collection of Listeria Institut Pasteur.
c
Negative, hly gene was not detected after 45 CT.
d
Strains of S. epidermidis lacking (CIP 68.21) or carrying (CIP 105.777) the Ica operon, which is essential for biofilm production.