Table 1.
Doubling times and cell yields of D. ethenogenes strain 195 using TCE and amended with 0 (AA0), 1 (AA1), 4 (AA4), or 20 (AA20) amino acids after five subcultures
| Culture | Mean ± SDa |
|
|---|---|---|
| Doubling time (days)b | Cell yield (107/μmol of Cl− released)c | |
| AA0 | 1.3 ± 0.3 | 3.8 ± 0.6 |
| AA1 | 0.95 ± 0.04 | 7.8 ± 0.3 |
| AA4 | 0.91 ± 0.16 | 10.0 ± 0.6 |
| AA20 | 0.76 ± 0.08 | 7.5 ± 2.5 |
a
F tests were performed to determine statistically significant differences (P < 0.05) in variances of the four conditions.
b
The doubling times were calculated from cell numbers during exponential growth on days 2 and 6 (tceA copies).
c
The Cl− released was computed using only metabolic dechlorination from TCE to VC as described by Duhamel and Edwards (6).