Fig. 5.

Detection of BoNT/E in avian blood samples using the BoTest Matrix E assay. Avian blood samples were tested for BoNT/E using the BoTest Matrix E assay as described in Materials and Methods. Immunoprecipitated BoNT/E was quantified using the BoTest E reporter (A and C) or the BoTest KO reporter (B and D) to control for nonspecific proteases. The resulting emission ratio for each sample was compared to a standard curve consisting of known amounts of BoNT/E (black circles). Representative data are shown from readings following a 2-h (A and B) or 8-h (C and D) incubation of capture BoNT/E with the reporters. LODs were calculated as described in Materials and Methods and indicated with dashed lines. Samples with emission ratio values below calculated LOD values were considered positive for BoNT/E.