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(A) SDS-PAGE analysis of heat extracts from isolates 8/02 and 251/01. Location of FliC (53 kDa) in the gel is indicated to the right. Western blot analysis of heat extracts (B), secreted proteins (C), or total protein extracts (D) of isolates using antiflagellin antibody. Fla−, a Salmonella isolate lacking flagella. (E) Fluorescent labeling of flagellar filaments in live cells of strain 8/02, 251/01, or PT4.
