Alignment of amino acid residues of MotA from several Gram-negative bacteria using ClustalW2. PT4, S. Enteritidis PT4 P125109; 251, S. Enteritidis isolate 251/01; LT2, S. Typhimurium strain LT2; Typhi, S. Typhi; Choler, S. enterica serovar Choleraesuis; Paraty, S. enterica serovar Paratyphi; Bpertu, Bordetella pertussis; Yerpes, Yersinia pestis; Ecoli, Escherichia coli. The Tm2 and Tm3 transmembrane segments are indicated with squares, and the mutated Val184 in 251/01 is shown in bold.