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PCR screening for motA(T551G) mutation. Colony-extracted DNA was used as a template in a PCR specific for wild-type (A) or motA(T551G) (B) alleles. Amplicon size is 113 bp. (A) PCR using primers motA2/motAWT2, amplifying only wild-type motA alleles. (B) PCR using primers motA2/motAmut2, amplifying only motA(T551G) alleles. Only a fraction of isolates analyzed are shown. Sample 7, isolate 18/97; 8, 19/97; 9, 20/97; 10, 21/97; 14, PT4; 15, 8/02; 16, 251/01.
