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Growth conditions. Growth in PYG medium at 18°C, 30°C, and 37°C was evaluated for individual survival of A. castellanii (A) and S. Typhi (B). The effect of growth medium composition of pure cultures of A. castellanii (C) or S. Typhi (D) incubated at 30°C was evaluated in PYG medium or diluted PYG (1/5-PYG) medium. Values are the means ± standard errors of the means of at least three independent experiments. Asterisks indicate a significant difference (P < 0.05).
