Table 2.
Oligonucleotides used in this study
| Primer | Sequence (5′ → 3′)a | Function |
|---|---|---|
| celB-qF1 | ATTTGGCCCGTGCTTACG | qRT-PCR, celB |
| celB-qR1 | TTTGGCAAACCTGCAAATAGG | |
| QptcC-F | CGTGTTCGGTATTGCTTACG | qRT-PCR, ptcC |
| QptcC-R | TGTTAAACCAGCGGGTACTC | |
| qBglS-F | TACACCGCAGTATGCTAAGG | qRT-PCR, bglS |
| qBglS-R | TTGGCCGACTTCAAGAGTTC | |
| Tuf-F | GGTAGTTGTCGAAGAATGGAGTGTGA | qRT-PCR, internal control |
| Tuf-R | TAAACCAGGTTCAATCACTCCACACA | |
| celB-1 | AACTCtAGATGGCCTTTGTA (XbaI) | Cloning and disruption of celB |
| celB-4 | gAagAtctAAGACAGCCGCTCC (BglII) |
a
Changes introduced to generate restriction sites (underlined and shown in parentheses) are indicated in lowercase.