Fig. 1.

Antioxidants impair sclerotial development in S. sclerotiorum. (A) Sclerotial initials generate large amounts of ROS. An agar-mycelium plug of the wild-type isolate 1980 was inoculated onto potato dextrose agar (PDA) and grown until the appearance of sclerotial initials. Aliquots of protoplasts generated from sclerotial initials were then stained with 2′,7′-dichlorodihydrofluorescein diacetate (DCHF-DA) to visualize ROS within the cells in response to N-acetyl-cysteine (NAC), as described in Materials and Methods. Images shown are representative of three independent experiments. (B) An agar-mycelium plug of the wild-type isolate 1980 was inoculated on PDA with or without 40 mM NAC or 1 mM diphenyleneiodonium (DPI). Sclerotial development was monitored and photographed.