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Expression analysis of Nox genes of S. sclerotiorum during development and plant infection. RNA was extracted from fungal and leaf tissues in the indicated stages, 4 days after inoculation with an agar plug (5 mm in diameter) colonized with S. sclerotiorum or uncolonized PDA. RT-PCR was performed with gene-specific primers for SsNox1, SsNox2, or elongation factor 1α (EF-1) as described in Materials and Methods. RT−, no-template control.
