Fig. 4.

Predicted and observed localizations of AGT-tagged proteins in D. vulgaris. Each column (left to right) depicts a representative image of an observed localization pattern in 10 proteins from D. vulgaris Hildenborough bearing chromosomally inserted visualization tags (AGT) at their respective C termini. Fluorescently labeled cells were imaged by deconvolution microscopy, and images in the table represent an optical section through the middle of the three-dimensional (3D) deconvolved image stack (20 to 30 sections along the z axis). Predicted localizations were obtained from PSORTb (www.psort.org/psortb/). PhsB (DVU0172), a predicted cytoplasmic protein, is uniformly distributed intracellularly. Proteins localizing exclusively at both cell poles include MotA (DVU2608) and ParA (DVU 3358). The FlgE (DVU1443) and UvrB (DVU1605) proteins localize at four distinct locations along the length of the cell. The Hup-3 (DVU1795) and PyrB (DVU2901) proteins show a patchy or spotty distribution. FtsH (DVU1278) localizes to the polar ends, in addition to having a dispersed cytoplasmic distribution. LytR (DVU0596) displays bipolar and midband localizations. MotA-1 (DVU0050) has its localization signal restricted to one polar end of the cell. A schematic representation of the observed localization pattern is shown in the inset. Scale bars represent 400 nm for images of PhsB and MotA and 500 nm for the rest of the images.