Fig. 1.
The spo0A regulatory region. (A) Cartoon of the regulatory region. Pv and Ps are indicated by black and blue boxes with arrows, respectively. Sequences matching the Spo0A consensus binding site (0A box), which are designated operator sites O1, O2, O3, and O4, are labeled with orange arrows and numbered in order from 5′ to 3′. The region extending from O1 to O3 (−192 to −143) is referred as O1–3 and the AT-rich region from position −142 to −99 (relative to the start codon) as “A-rich.” Regions deleted in mutants are indicated by pink bars with corresponding mutant names. (B) Sequence of the regulatory region. The start sites of transcription (+1) for the Pv and Ps promoters are underlined and highlighted by a vertical arrow. Positions are numbered relative to the initiation codon GTG (indicated in bold). Sequences matching 0A boxes are indicated with orange arrows and numbered as in panel A. Brackets indicate limits of the deletion in ΔPv (Abs894), ΔO1–3 (Abs883), ΔPv ΔO1–3 (Abs875), or ΔPs (Abs786).