Table 1.
B. subtilis strains used in this study
| Strain | Genotype | Source or referencea |
|---|---|---|
| CU4121 | purM::Tn917 (erm) trpC2 | BGSC |
| PS251 | codY::(erm::spc) trpC2 | P. Serror |
| SMY | Prototroph | 44 |
| YXJAd | nupG::pMutin1 (erm) | NBRP |
| NUPNd | nupN::pMutin2 (erm) | NBRP |
| NUPOd | nupO::pMutin2 (erm) | NBRP |
| BB1888 | lacA::tet | 6 |
| BB2511 | ΔamyE::spc lacA::tet | 6 |
| BB2686 | ΔamyE::[erm Φ(nupN503p+-lacZ)]lacA::tet | BB2511 × pBB1474 |
| BB2809 | ΔamyE::[erm Φ(nupN276p+-lacZ)]lacA::tet | BB2511 × pBB1520 |
| BB2899 | ΔamyE::[erm Φ(nupN347p+-lacZ)]lacA::tet | BB2511 × pBB1521 |
| BB2900 | ΔamyE::[erm Φ(nupN120p+-lacZ)]lacA::tet | BB2511 × pBB1522 |
| BB2935 | ΔamyE::[erm Φ(nupN367p+-lacZ)]lacA::tet | BB2511 × pBB1534 |
| BB2936 | ΔamyE::[erm Φ(nupN140p+-lacZ)]lacA::tet | BB2511 × pBB1535 |
| BB2952 | ΔamyE::[erm Φ(nupN367p1-lacZ)] lacA::tet | BB2511 × pBB1542 |
| BB2953 | ΔamyE::[erm Φ(nupN380p+-lacZ)]lacA::tet | BB2511 × pBB1543 |
| BB2954 | ΔamyE::[erm Φ(nupN140p1-lacZ)] lacA::tet | BB2511 × pBB1544 |
| BB2955 | ΔamyE::[erm Φ(nupN153p+-lacZ)]lacA::tet | BB2511 × pBB1545 |
| BB2972 | ΔamyE::[erm Φ(nupN380p21-lacZ)] lacA::tet | BB2511 × pBB1554 |
| BB2973 | ΔamyE::[erm Φ(nupN380p22-lacZ)] lacA::tet | BB2511 × pBB1555 |
| BB2974 | ΔamyE::[erm Φ(nupN153p21-lacZ)] lacA::tet | BB2511 × pBB1556 |
| BB2975 | ΔamyE::[erm Φ(nupN153p22-lacZ)] lacA::tet | BB2511 × pBB1557 |
| BB3014 | ΔamyE::[erm Φ(nupN153p2-lacZ)] lacA::tet | BB2511 × pBB1574 |
| BB3015 | ΔamyE::[erm Φ(nupN153p3-lacZ)] lacA::tet | BB2511 × pBB1575 |
| BB3322 | ΔnupN amyE::spc lacA::tet | BB2511 × pBB1654 |
| BB3401 | ΔamyE::[erm Φ(nupN347p11-lacZ)] lacA::tet | BB2511 × pBB1676 |
| BB3402 | ΔamyE::[erm Φ(nupN503p12-lacZ)] lacA::tet | BB2511 × pBB1677 |
| BB3431 | ΔamyE::[erm Φ(nupN380p3/11-lacZ)] lacA::tet | BB2511 × pBB1691 |
| BB3484 | ΔnupN amyE::spc lacA::tet purM::Tn917 (erm) | BB3322 × CU4121 DNA |
| BB3486 | ΔnupN nupG::pMutin1 (erm) amyE::spc lacA::tet | BB3332 × YXJAd DNA |
| BB3491 | ΔamyE::[erm Φ(nupN380p3-lacZ)] lacA::tet | BB2511 × pBB1698 |
| BB3492 | ΔamyE::[erm Φ(nupN380p11-lacZ)] lacA::tet | BB2511 × pBB1699 |
| BB3493 | ΔamyE::[erm Φ(nupN347p12-lacZ)] lacA::tet | BB2511 × pBB1700 |
| BB3499 | ΔnupN nupG::pMutin1 (erm) amyE::spc lacA::tet purM::(Tn917::neo) | BB3485 × YXJAd DNA |
| BB3501 | nupG::pMutin1 (erm) lacA::tet | BB1888 × YXJAd DNA |
| BB3502 | nupG::pMutin1 (erm) lacA::tet purM::(Tn917::neo) | BB3501 × BB3499 DNA |
| BB3546 | purM::(Tn917::neo) | SMY × BB3485 DNA |
| BB3597 | ΔamyE::[erm Φ(nupN153p23-lacZ)]lacA::tet | BB2511 × pBB1724 |
| BB3598 | ΔamyE::[erm Φ(nupN153p24-lacZ)]lacA::tet | BB2511 × pBB1725 |
| BB3599 | ΔamyE::[erm Φ(nupN153p2/21-lacZ)]lacA::tet | BB2511 × pBB1726 |
a
BSCG, Bacillus Genetic Stock Center; NBRP, National BioResource Project (NIG, Japan): B. subtilis (http://www.shigen.nig.ac.jp/bsub/). × denotes transformation by plasmid or chromosomal DNA.