Table 4.

Expression of nupN-lacZ fusions^a

Strain	Relevant genotype	Fusion genotype	Motifs present	Growth medium
				TSS + 16 amino acids		TSS
				β-Galactosidase activity (MU)	Repression ratio	β-Galactosidase activity (MU)	Repression ratio
BB2953	Wild type	nupN380p+	1b, 1, 2	0.91	381	171.0	2.7
BB2960	codY			347.0		468.0
BB3491	Wild type	nupN380p3	1b, 1down, 2	0.78	105	67.1	1.2
BB3495	codY			81.8		77.4
BB3492	Wild type	nupN380p11	1b, 1, 2up	0.20	1,525	88.8	5.3
BB3496	codY			305.0		471.0
BB3431	Wild type	nupN380p3/11	1b, 1down, 2up	0.77	326	166.0	2.7
BB3435	codY			251.0		449.0
BB2972	Wild type	nupN380p21	1bup, 1, 2	0.38	982	28.0	18
BB2976	codY			373.0		492.0
BB2973	Wild type	nupN380p22	1bdown, 1, 2	9.25	41	377.0	1.3
BB2977	codY			379.0		496.0
BB2955	Wild type	nupN153p+	1b, 1	0.09	174	4.01	2.4
BB2962	codY			15.7		9.71
BB3014	Wild type	nupN153p2	1b, 1down	2.27	1.6	2.07	0.60
BB3019	codY			3.54		1.24
BB3015	Wild type	nupN153p3	1b, 1down	2.41	6.8	12.5	0.81
BB3020	codY			16.3		10.1
BB2974	Wild type	nupN153p21	1bup, 1	0.04	423	0.27	47
BB2978	codY			16.9		12.8
BB2975	Wild type	nupN153p22	1bdown, 1	1.30	13	12.3	0.97
BB2979	codY			17.0		11.9
BB3597	Wild type	nupN153p23	1bdown, 1	9.30	1.5	8.96	0.82
BB3600	codY			13.6		7.36
BB3598	Wild type	nupN153p24	1bdown, 1	0.16	99	6.14	1.7
BB3601	codY			15.9		10.3
BB3599	Wild type	nupN153p2/21	1bup, 1down	0.09	43	1.14	0.80
BB3602	codY			3.91		1.42
BB2935	Wild type	nupN367p+	1, 2	29.8	8.0	396.0	0.93
BB2943	codY			238.0		368.0
BB2952	Wild type	nupN367p1	1up, 2	0.31	623	30.6	8.2
BB2959	codY			193.0		250.0
BB2936	Wild type	nupN140p+	1	12.9	0.95	12.3	0.78
BB2944	codY			12.3		9.64
BB2954	Wild type	nupN140p1	1up	0.05	172	0.37	16
BB2961	codY			8.60		5.88

^aCells were grown, β-galactosidase activity was assayed, and repression ratios were calculated as described in Table 3.