Fig. 3.
Phenotypes of S. coelicolor M851+pIJ6902-hyg (adpA deletion mutant) and M851+adpA(TTG) compared to the wild-type S. coelicolor M145+pIJ6902-hyg. Cultures were grown on R2 medium (supplemented with 2 μg ml−1 thiostrepton) for 48 h. The levels of expression of AdpA protein in the analyzed S. coelicolor strains are shown in the blot at the bottom of the figure. Western blot analysis of total S. coelicolor cell proteins (40 μg protein per lane) was performed using rabbit antibodies raised against the C terminus of recombinant AdpASc protein.