Table 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Descriptiona | Source or reference |
|---|---|---|
| Strains | ||
| E. coli | ||
| DH5α | F′ φ80dlacZΔM15 endA1 recA1 hsdR17(rK− mK+) supE44 thi-1 Δ(lacZYA-argF)U169 deoR λ− | Invitrogen |
| BL21(DE3) | F′ dcm ompT hsdS(rB− mB−) galDE3(lacI lacUV5-T7 gene 1 ind-1 sam-7 nin-5) | Novagen |
| C. jejuni | ||
| NCTC11168 | Wild type, a human isolate | 27 |
| FMB3001 | pMW10::PperR/NCTC11168 | This study |
| FMB3003 | perR mutant (perR::cat) | This study |
| FMB3007 | pMW10::PperR/FMB3003 | This study |
| FMB3008 | perR complementation | This study |
| Plasmids | ||
| pUC19 | Cloning and suicide vector; Ampr | New England Biolabs |
| pUC19-perR | pUC19 carrying perR | This study |
| pUC19-perR::cat | pUC19 carrying perR::cat | This study |
| pET15b | Ampr; N-terminal His tag, T7 promoter, pBR322 ori | Novagen |
| pET15b-perR | pET15b carrying perR | This study |
| pMW10 | E. coli-C. jejuni shuttle plasmid; lacZ mob repB; Kanr | 36 |
| pMW10::PperR | pMW10 carrying perR promoter | This study |
| pMW10::PperR-perR | pMW10 carrying perR promoter and perR | This study |
| pRY112 | E. coli-C. jejuni shuttle plasmid; Cmr | 37 |
a
Cmr, chloramphenicol resistance; Ampr, ampicillin resistance; Kanr, kanamycin resistance.