Table 2.
Primers and probes used in this study
| Type of analysis and primer or probe | Sequence (5′-3′)a |
|---|---|
| RT-PCR | |
| MR-F | CCAAGAAGCAGCGCAACCAAT |
| MR-R | GTAGTAGTAGACGGCCGATAT |
| Construction and verification of mdoR deletion mutant | |
| L1-F | AGGTAGCGTCCATGACAGATA |
| L1-R | tctagaCACTTCTCGTCGATCGTGGAT* |
| R1-F | tctagaAGCCACACCGATTCCGATGAT* |
| R1-R | tctagaAATAGTGCAGGGATTCAAGCG* |
| MRid-F | AATGGGCCGCGAAGAACTGCG |
| MRid-R | TACAACGTACGAATGCAGGTA |
| Construction of MdoR expression plasmid | |
| MdoR-F | gaattcCATGGGTACCGCATCATCGGA† |
| MdoR-R | ctgcagCGGCTTCAGGTTGCCGATGA‡ |
| Construction of mdoR complementation plasmid | |
| CR-F | ctgcagGATCTACGGCTTCAGGTTGCC‡ |
| CR-R | ctgcagATCCAACAAGGTGGTCGCCGC‡ |
| DNase I footprinting | |
| RF-F | gaattcAGCTTGCGCATGCTCAGCCCG† |
| RF-R | ctgcagACGCGAAGCCCGCATCCAACA‡ |
| Construction of reporter plasmid | |
| RP-F | tctagaATTGGTTGCGCTGCTTCTTGG* |
| RP-R | atcgatTCGTCGATCGTCGTGTCGCAG§ |
| EMSAb | |
| MDP-F | CGGGCAGCGTGCTGCGAGT |
| MDP-R | ACTCGCAGCACGCTGCCCG |
| MRP-F | CCTCCGTACGCTGTACGTAATGCAACGTACAACGTACGAAT |
| MRP-R | ATTCGTACGTTGTACGTTGCATTACGTACAGCGTACGGAGG |
| MRIR1-F | CCTCCGTGTGCTGTACGTAATGCAACGTACAACGTACGAAT |
| MRIR1-R | ATTCGTACGTTGTACGTTGCATTACGTACAGCACACGGAGG |
| MRIR2-F | CCTCCGTACGCTGTACGTAATGCAACGTGTAACGTACGAAT |
| MRIR2-R | ATTCGTACGTTACACGTTGCATTACGTACAGCGTACGGAGG |
| MRIR12-F | CCTCCGTGTGCTGTACGTAATGCAACGTGTAACGTACGAAT |
| MRIR12-R | ATTCGTACGTTACACGTTGCATTACGTACAGCACACGGAGG |
a
*, the XbaI recognition sequence is indicated in lowercase, underlined text; †, the EcoRI recognition sequence is indicated in lowercase, underlined text; ‡, the PstI recognition sequence is indicated in lowercase, underlined text; §, the ClaI recognition sequence is indicated in lowercase, underlined text.
b
For the EMSA sequences, underlining indicates the inverted repeat sequences. Boldface italics within the (underlined) inverted repeat sequences indicates the base transversion of the inverted repeat sequences CGTAC and GTACG.