Table 1.
B. subtilis strains and plasmids used in this study
| Strain or plasmid | Genotype or description | Construction or reference |
|---|---|---|
| Strains | ||
| 168 | trpC2 | Lab strain |
| BSU2007 | 168 ΔsigMWXYVZ ΔylaC (Δ7ECF) | 1 |
| HB12010 | BSU2007 amyE::PxylA-sigV | pVG001 → BSU2007 |
| HB12020 | BSU2007 amyE::PxylA-sigW | pVG003 → BSU2007 |
| HB12035 | BSU2007 amyE::PxylA-sigM | pVG0013 → BSU2007 |
| HB12036 | BSU2007 amyE::PxylA-sigX | pVG004 → BSU2007 |
| HB12027 | 168 sigV::kan | LFH-PCR → 168 |
| HB12082 | 168 amyE::PxylA-sigV | pVG001 → 168 |
| HB0048 | CU1065 dltA::spc | 19 |
| HB12093 | 168 dltA::spc | HB0048 ChrDNAa → 168 |
| HB12083 | 168 oatA::tet | LFH-PCR → 168 |
| HB12092 | 168 oatA::tet dltA::spc | HB12083 ChrDNA → HB-12093 |
| HB12129 | 168 sigV::kan oatA::tet dltA::spc | HB12027 ChrDNA → HB-12092 |
| HB10016 | 168 sigM::tet | 44 |
| HB10102 | 168 sigW::mls | 44 |
| HB10103 | 168 sigX::kan | 44 |
| HB10107 | 168 sigM::tet sigX::kan sigW::mls (Δ3) | 44 |
| Plasmids | ||
| pVG001 | pSWEET-PxylA-sigV (Cmr) | This work |
| pVG003 | pSWEET-PxylA-sigW (Cmr) | This work |
| pVG004 | pSWEET-PxylA-sigX (Cmr) | This work |
| pVG0013 | pSWEET-PxylA-sigM (Cmr) | This work |
a
ChrDNA, chromosomal DNA.