Table 2.
Plasmids used in this study
| Plasmid | Descriptiona | Source or reference |
|---|---|---|
| pKD4 | FRT-kan-FRT template, Ampr | 9 |
| pKD13 | FRT-kan-FRT template, Ampr | 9 |
| pKD46 | Phage Red expression plasmid, Ampr | 9 |
| pCP20 | Flp expression plasmid, Ampr Catr | 9 |
| pSUB11 | 3×FLAG tag FRT-kan-FRT template, Ampr | 44 |
| pKK1064 | pBR322 fliB-IS200-fliA-fliZ′ | 39 |
| pBAD18kan | ParaBAD expression vector, Kanr | 11 |
| pBAD-P1-fliA | pBAD18kan fliA (−155 to +720), P2 negative (−33CGT−31) | This study |
| pQE80L | His6-tagged protein expression vector, Ampr | Qiagen |
| pQE-fliA | pQE80L fliA (+1 to +720), His6-FliA | This study |
| pTrc99A | Ptrc expression vector, Ampr | 2 |
| pTrc99K | pTrc99A amp::kan, Amps Kanr | Laboratory stock |
| pTrc-fliA | pTrc99K fliA (−19 to +720) | This study |
| pTrc-P1/P2-fliA | pTrc99K fliA (−115 to +720) | This study |
| pTrc-P2-fliA | pTrc99K fliA (−57 to +720) | This study |
| pTrc-P2c-fliA | pTrc99K fliA (−57 to +720), P2 consensus (−52TAAAGTTT−45) | This study |
| pFZY1 | Transcriptional lacZ fusion vector, Ampr | 23 |
| pF-P1/P2 | pFZY1 fliA (−115 to +399) | pFZY1-PfliA-lacZ in reference 46 |
| pF-P1/P2-fliA | pFZY1 fliA (−115 to +720) | This study |
| pF-P1-fliA | pFZY1 fliA (−115 to +720), P2 negative (−33CGT−31) | This study |
| pF-P1(P2)-fliA | pFZY1 fliA (−115 to +720), −29 to −19 deleted | This study |
| pF-P2-fliA | pFZY1 fliA (−57 to +720) | This study |
| pF-P2c-fliA | pFZY1 fliA (−57 to +720), P2 consensus (−52TAAAGTTT−45) | This study |
| pF-P2(P1)-fliA | pFZY1 fliA (−57 to +720), −29 to −19 duplicated | This study |
| pF-P1/P2-fliAZ | pFZY1 fliA (−155)-fliZ (+552) | This study |
| pF-P1-fliAZ | pFZY1 fliA (−155)-fliZ (+552), P2 negative (−33CGT−31) | This study |
| pF-P1(P2)-fliAZ | pFZY1 fliA (−155)-fliZ (+552), −29 to −19 deleted | This study |
| pF-P2-fliAZ | pFZY1 fliA (−57)-fliZ (+552) | This study |
| pF-P2(P1)-fliAZ | pFZY1 fliA (−57)-fliZ (+552), −29 to −19 duplicated | This study |
| pMC1403 | Translational lacZ fusion vector, Ampr | 5 |
| pMC-P1 | pMC1403 fliA (−115 to +398), P2 negative (−33CGT−31) | This study |
| pMC-P1(P2) | pMC1403 fliA (−115 to +398), −29 to −19 deleted | This study |
| pMC-P1(GC*) | pMC1403 fliA (−115 to +398), P2 negative (−33CGT−31), GC* (−28CGG−26) | This study |
| pMC-P2 | pMC1403 fliA (−57 to +398) | This study |
| pMC-P2(P1) | pMC1403 fliA (−57 to +398), −29 to −19 duplicated | This study |
| pMC-P2(SD*) | pMC1403 fliA (−57 to +398), 5′ UTR from pQE80L | This study |
a
The DNA regions inserted in the plasmids are shown with the translation initiation codon of the fliA or fliZ gene being numbered 1.