Table 5.
Transcriptional activity from the fliA promoter on a single-copy plasmid
| Host genotypea |
Expression plasmid | LacZ expression (Miller units)b |
||||
|---|---|---|---|---|---|---|
| flhD | flgM | fliA | fliZ | pF-P1/P2 | pF-P1/P2-fliA | |
| + | + | + | + | − | 330 ± 35 | 290 ± 37 |
| + | − | + | + | − | 860 ± 85 | 1,300 ± 120 |
| + | + | − | + | − | 17 ± 6.4 | 120 ± 64 |
| + | − | − | + | − | 9.8 ± 1.5 | 770 ± 45 |
| − | + | + | + | − | 0.0 ± 1.4 | 1.2 ± 1.3 |
| − | − | + | + | − | 0.35 ± 1.9 | 6.6 ± 0.84 |
| + | + | + | − | − | 6.9 ± 0.61 | 8.0 ± 1.6 |
| + | − | + | − | − | 27 ± 7.4 | 77 ± 23 |
| − | + | + | + | pTrc99K | 0.0 ± 0.11 | ND |
| − | + | + | + | pTrc-fliA | 6.0 ± 1.2 | ND |
| − | − | + | + | pTrc99K | 0.24 ± 0.18 | ND |
| − | − | + | + | pTrc-fliA | 640 ± 23 | ND |
a
The flhD, flgM, fliA, and fliZ mutations used originated from KK1004hDC, KK1004gM, KK1004iA, and KK1004iZ, respectively.
b
In pF-P1/P2, the lacZ gene with its own SD sequence follows the P1 and P2 promoters of the fliA gene. pF-P1/P2-fliA has the same structure as pF-P1/P2, except that it contains an intact fliA gene. Cells grown in MGC were used. ND, not done.