Table 7.
Translational activity of the altered versions of fliA mRNA
| Translational fusion plasmid |
Host genotypea | LacZ expression (Miller units)b | ||
|---|---|---|---|---|
| Name | Promoter | 5′ UTRc | ||
| pMC-P1 | P1 | P1 | ΔfliAZ | 1,300 ± 44 |
| pMC-P1(P2) | P1 | P2 | ΔfliAZ | 3.1 ± 0.57 |
| pMC-P1(GC*) | P1 | P1(GC*) | ΔfliAZ | 23 ± 1.5 |
| pMC-P2 | P2 | P2 | ΔflgM | 33 ± 1.5 |
| pMC-P2(P1) | P2 | P1 | ΔflgM | 1,000 ± 160 |
| pMC-P2(SD*) | P2 | P2(SD*) | ΔflgM | 4,700 ± 1,600 |
a
Host strains used were KK1004iAZ for ΔfliAZ and KK1004gM for ΔflgM.
b
Cells grown in MGC were used.
c
GC*, the AT-rich sequence of the 5′ end of P1 mRNA was altered to a GC-rich sequence; SD*, the 5′ UTR sequence of the fliA gene was replaced with a sequence identical to the 5′ UTR sequence of the His6-tagged fusion protein gene on pQE80L, resulting in substitution of the SD sequence of the fliA gene (AGG) with a stronger one (AGGAG).