Fig. 4.

Percentage of GFP-positive cells infected by the indicated B. henselae mutant strains carrying plasmid pHP161 (oriT trwABC) (panel A) or wild-type B. henselae carrying plasmids which expressed the indicated TrwB or TrwC variants (panel B). The bars represent means with standard deviation from at least three independent experiments done in triplicate. Strains in panel A are identified by the deleted trw-Bt or vir genes, e.g., the ΔB2-11 is the strain with a virB2-to-virB11 deletion, and so on. Plasmids in panel B are named by their difference from pHP161, containing wild-type trwABC genes, as follows: ABC, wild-type plasmid; ΔB and ΔC, deletion of trwB and trwC, respectively; C::BID, TrwC-BID fusion protein; TrwB and TrwC point mutants are indicated.