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Ax21 secretion is dependent on the RaxB homolog Smlt2001, a putative ABC transporter ATP binding protein. The level of Ax21 activity in culture supernatants from selected strains grown in NYGB medium to an OD at 600 nm of 2.0 was assessed by a bioassay in which the restoration of motility to an ax21 (smlt0387) mutant was measured.
