Loss of one Gata-3 allele reduces tumor latency and affects tumor morphology. (A) Kaplan-Meier tumor-free survival curves of PyMT; Gata-3+/+ and PyMT; Gata-3+/nlslacZ mice. The median tumor-free survival times were 78 days versus 48 days, respectively (P < 0.0001). (B) Western blot analysis showing expression level of Gata-3 in three representative PyMT; Gata-3+/+ and PyMT; Gata-3+/nlsLacZ mammary tumors. (C) Hematoxylin and eosin (scale bars, 200 μm) and immunofluorescence staining for NPt2b in mammary lesions arising in 7-week-old PyMT; Gata-3+/+ and PyMT; Gata-3+/nlslacZ mice (scale bars, 100 μm). (D) Whole-mount (scale bars, 2 mm) and hematoxylin and eosin (scale bars, 200 μm) sections of tumors arising from transplanted unsorted cells (160 cells) from 5- to 6-week-old PyMT; Gata-3+/+ and PyMT; Gata-3+/nlslacZ mice.