Table 1.

Primer sequences used in this study

Primer use	Primer sequencesa
Genotyping
MTB	Fwd, 5′-TGCCGCCATTATTACGACAAGC-3′; Rev, 5′-ACCGTACTCGTCAATTCCAAGGG-3′
Gata-3+/Tg	Fwd, 5′-ACGTCTCACTCTCGAGGCAGCATG-3′; Rev, 5′-GAAGTCCTCCAGCGCGTCATGCAC-3′
Gata-3+/nlslacZ	Fwd, 5′-CAGGAGTCCGCGGACCTCC-3′; Rev, 5′-CGTTGAGGACCGCGGGGTG-3′; Rev, 5′-CCAAGCTTGGACTCAAAAAAC-3′
MMTV-PyMT	Fwd, 5′-CTCTAGAGGATCTTTGTGAAGG-3′; Rev, 5′-GGACAAACCACAACTAGAATGC-3′
Gene expression analysis
β-Casein	Fwd, 5′-AAAGGACTTGACAGCCATGAA-3′; Rev, 5′-TAGCCTGGAGCACATCCTCT-3′
Gata-3	Fwd, 5′-AGCCACATCTCTCCCTTCAG-3′; Rev, 5′-AGGGCTCTGCCTCTCTAACC-3′
Caspase-14	Fwd, 5′-GATGAGGTTGCTGTGCTCAA-3′; Rev, 5′-GAACACATCCGTCAGGGTCT-3′
GAPDH	Fwd, 5′-GTATTGGGCGCCTGGTCACC-3′; Rev, 5′-CGCTCCTGGAAGATGGTGATGG-3′
18S rRNA	Fwd, 5′-GTAACCCGTTGAACCCCATT-3′; Rev, 5′-CCATCCAATCGGTAGTAGCG-3′
Chromatin immunoprecipitation
Control	Fwd, 5′-AGGCATCTCTTTGAGATGGCT-3′; Rev, 5′-TGGGTCATTCACCAGCTTCT-3′
−484	Fwd, 5′-CTGTCTGTGCAGCCAGAGAA-3′; Rev, 5′-AGCAGGGCAAGTGACCCATGTG-3′
−3060	Fwd, 5′-CCTTATAGGGTTGAATGCCAA-3′; Rev, 5′-TGGCCTAGGCAGAGAGTCT-3′
−3772	Fwd, 5′-GACTGGCAGGTAGGGAAGGTGAG-3′; Rev, 5′-ATAGTGAAACCAAAGGCGAAC-3′
−4392	Fwd, 5′-ATCTCACACTTTGGGAGGCCGAGTG-3′; Rev, 5′-ACTCGGTTCGATAGCAACAGCT-3′
Plasmid construction
Gata-3 Tg (pTet-On-Gata-3)	Fwd, 5′-GGAAGATCTCACCATGGACTACAAAGACGATGACGATAAAGAGGTGACTGCGGACCAGCCGCGC-3′; Rev, 5′-GGAAGATCTCTAACCCATGGCGGTGACCAT-3′
pFU-TA-GFP Gata-3	Fwd, 5′-TGCGAATTCATGGAGGTGACGGCGGACCAGCCGCGC-3′; Rev, 5′-CCGAGATCTACCCATGGCGGTGACCATGCTGGA-3′
pFU-TA-GFP caspase-14	Fwd, 5′-CGCGAATTCTCACCATGAGCAATCCGCGGTCTT-3′; Rev, 5′-CCGGGATCCCTGCAGATACAGCCGTTTCCGGA-3

^aFwd, forward; Rev, reverse.