Table 2.
Limiting dilution analysis of the repopulating frequency of CD29hi CD24+ MaSC-enriched cells from control or MMTV-cre; Gata-3f/f mammary glandsa
| Recipient | No. of CD29hi CD24+ cells injected per mammary fat pad | No. of positive outgrowthsb |
|
|---|---|---|---|
| Gata-3+/f | MMTV-cre; Gata-3f/f | ||
| Rag1−/− | 75 | 4/9 | 0/9 |
| 150 | 1/9 | 1/9 | |
| 250 | 8/11 | 2/10 | |
| 500 | 7/8 | 3/8 | |
| Repopulating frequency (95% confidence interval) | 1/254 (1/406–1/159) | 1/1,232 (1/2,737–1/554) | |
| P value | 0.0003 | ||
| NOD-SCID | 250 | 2/2 | 1/2 |
| 500 | 3/4 | 0/4 | |
| Repopulating frequency (95% confidence interval) | 1/244 (1/670–1/87) | 1/2,373 (1/17,594–1/320) | |
| P value | 0.016 | ||
a
Limiting dilution analysis of the repopulating frequency of CD29hi CD24+ cells (double sorted) from the mammary glands of Gata-3+/f or MMTV-cre;Gata-3f/f mice. Cells were injected into the cleared mammary fat pads of 3-week-old Rag1−/− or NOD-SCID immunocompromised recipients and fat pads collected 8 weeks posttransplantation. Data are pooled from three (Rag1−/−) or two (NOD-SCID) independent experiments for each cell type.
b
Shown as number of outgrowths per number of injected fat pads.