Table 3.
Frequency of tumor-initiating cells in mammary gland lesions from PyMT; Gata-3+/+ or PyMT; Gata-3+/nlslacZ micea
| No. of cells injected per mammary fat pad | No. of tumorsb |
|
|---|---|---|
| PyMT; Gata-3+/+ | PyMT; Gata-3+/nlslacZ | |
| 10 | 1/12 | 0/12 |
| 40 | 0/6 | 1/6 |
| 160 | 6/12 | 8/12 |
| 640 | 7/10 | 10/10 |
| 2,560 | 11/12 | 12/12 |
| 10,240 | 5/5 | 6/6 |
| Tumor-initiating frequency (95% confidence interval) | 1/556 (1/972–1/318) | 1/156 (1/275–1/89) |
| P value | 0.001 | |
a
Limiting dilution analysis of the tumor-initiating frequency of unsorted mammary gland cells isolated from PyMT; Gata-3+/+ or PyMT; Gata-3+/nlslacZ mice at 5 to 6 weeks of age. Cells were injected into the cleared mammary fat pads of 3-week-old syngeneic FVB/N recipients and fat pads collected 10 weeks posttransplantation. Data are shown for two independent experiments.
b
Shown as number of outgrowths per number of injected fat pads.