Table 4.
Frequency of tumor-initiating cells in GFP subpopulations isolated from PyMT; MTB; Gata-3+/Tg mammary glandsa
| No. of cells injected per mammary fat pad | No. of tumorsb |
|
|---|---|---|
| Lin− CD24+ GFP− | Lin− CD24+ GFP+ | |
| 200 | 4/14 | 0/14 |
| 400 | 8/20 | 3/20 |
| 800 | 5/6 | 1/6 |
| Repopulating frequency (95% confidence interval) | 1/648 (1/1,051–1/399) | 1/3,643 (1/9,704–1/1,368) |
| P value | 0.0004 | |
a
Limiting dilution analysis of the tumor-initiating frequency of Lin− CD24+ GFP− and Lin− CD24+ GFP+ cells isolated from PyMT; MTB; Gata-3+/Tg mice. Cells were injected into the cleared mammary fat pads of 3-week-old syngeneic recipient mice and fat pads collected 10 weeks posttransplantation. Data are shown for three independent experiments.
b
Shown as the number of tumors per number of injected fat pads. For transplanted GFP+ cells, only GFP-positive tumors were counted.