Table 2.
Selected potential CTL epitope peptides from the 2009 pH1N1 influenza A virus, based on the SLA-1*0401 binding motif
| Protein and position | Amino acid sequence | Published MHC allelea | Published CTL response (reference[s])a | Stability with SLA-1*0401b |
|---|---|---|---|---|
| NA | ||||
| 25–33 | QIGNIISIW | / | / | ++ |
| 266–274 | SVEMNAPNY | / | / | ++ |
| 414–423 | GLDCIRPCFW | / | / | ++ |
| HA | ||||
| 16–24 | NADTLCIGY | / | / | ++ |
| 343–351 | IAGFIEGGW | B58 | / | −c |
| 358–366 | WTGMVDGWY | A1 | / | − |
| 445–454 | LLENERTLDY | / | / | ++ |
| M1 | ||||
| 6–15 | EVETPTRSEW | / | / | ++ |
| 36–45 | NTDLEALMEW | / | / | ++ |
| M2 | ||||
| 83–91 | AVDVDDGHF | / | / | ++ |
| NP | ||||
| 9–17 | MIGGIGRFY | / | / | +d |
| 44–52 | CTELKLSDY | A1 | Positive (3, 10, 12) | ++ |
| 378–386 | TLELRSRYW | / | / | ++ |
| NS1 | ||||
| 194–202 | VSENIQRFAW | / | / | ++ |
| PA | ||||
| 437–445 | HIASMRRNY | / | / | ++ |
| 531–539 | RLEPHKWEKY | / | / | ++ |
| PB1 | ||||
| 347–355 | KMARLGKGY | A1(23), A3, A26, B8, B27, B58, B62 | Positive (72) | + |
| 372–380 | MLASIDLKY | A1, Mamu-A*02(23) | − | |
| 542–551 | ATAQMALQLF | Mamu-A*01, Mamu-A*02 | / | + |
| 591–599 | VSDGGPNLY | A1, A26, A80, B15, B18, B58, Mamu-A*02 | Positive (2, 12) | ++ |
| PB2 | ||||
| 197–205 | KIAPLMVAY | / | / | ++ |
| 213–222 | VAGGTGSVY | / | / | + |
a
/, no information available.
b
++, peptide binds strongly and can tolerate anion-exchange chromatography; −, peptide does not bind SLA-1*0401; +, peptide binds SLA-1*0401 but cannot tolerate anion-exchange chromatography.