Fig. 6.

WNV-specific antibody responses in viperin^−/− mice. Wild-type and viperin^−/− mice were infected with 10² PFU of WNV by subcutaneous injection into the footpad. At the indicated times postinfection, serum was collected and assayed by ELISA for WNV E-specific IgM (A) and IgG (B) antibody titers. Data represent the reciprocal endpoint titers. (C) Sera from the indicated times postinfection were tested for neutralization activity using a focus reduction assay. Data represent the effective concentration that produced 50% neutralization of WNV infection (EC₅₀). Asterisks indicate values that are statistically significant (*, P < 0.05; **, P < 0.005).