Fig. 7.

WNV replication in regions of the CNS of viperin^−/− mice after intracranial infection. Viperin^−/− and wild-type mice were infected with 10¹ PFU WNV via intracranial injection. At the indicated times postinfection, cerebral cortex (gray matter) (A), white matter (B), brain stem (C), cerebellum (D), and spinal cord (E) were harvested and analyzed for viral burden by plaque assay on BHK21-15 cells. Data are shown as PFU per gram of tissue for 7 to 17 mice per time point. Solid lines represent the median viral titers, and dotted lines indicate the limit of detection of the respective assays. Asterisks indicate values that are statistically significant (*, P < 0.05; **, P < 0.005).