Table 1.
2F5 VH2-5 HCDR2 UA variants do not neutralize HIV-1a
| Antibody | IC50 (μg/ml) |
||
|---|---|---|---|
| BG1168 | SF162 | MN | |
| 2F5 MAb | 2.347 | 1.163 | 0.103 |
| RUA variant 1 (DH54) | >50 | >50 | >50 |
| RUA variant 2 (NH54) | >50 | >50 | >50 |
a
HIV-1 pseudovirus neutralization was determined by reduction of luciferase reporter gene expression after a single round of infection by pseudotyped HIV-1 viruses in TZM-bl cells. The pseudotyped viruses containing Envs derived from HIV-1 isolates BG1168.B, SF162.B, and MN.B were preincubated with serial dilutions (3-fold) of the antibodies (150 μg/ml) in 96-well plates. After mixing and incubation with target TZM-bl cells, the concentration of half-maximal inhibition (IC50) was calculated from the luciferase activities determined by luminescence measurements. The pseudotyped virus murine leukemia virus (MLV-SVA), which is not neutralized by 2F5, was used as a control.