Fig. 1.
Gel overlay assays detect interactions between Bean dwarf mosaic virus (BDMV) movement proteins (NSP and MP) and plant proteins. (A) Wild-type and BDMV MP transgenic tomato plants. Expression of MP resulted in disease symptom development. (B) Protein profiles obtained for wild-type (Wt) and MP-expressing (T) tomato leaves. Proteins were fractionated (P1, P30, P0, and S30), separated by SDS-PAGE, and visualized by Coomassie brilliant blue staining. CH, core histones from chicken erythrocytes. (C) Gel overlays of host protein fractions probed with 35S-labeled in vitro-translated NSP (NSP-P) or MP (MP-P) and Western blot analysis of equivalent fractions probed with MP antiserum (MP-Ab).