Fig. 3.
The Bean dwarf mosaic virus (BDMV) NSP and histone H3 proteins interact with capsid proteins (CPs) from a range of geminiviruses but not with the CP of an RNA virus. Purified recombinant CPs of Tomato yellow leaf curl virus (TYLCV), Beet mild curly top virus (BMCTV), BDMV, and TMV were separated by SDS-PAGE and visualized by Coomassie brilliant blue (CBB) staining. Proteins were transferred to PVDF membranes and probed with 35S-labeled in vitro-translated NSP or H3. Recombinant histone H3 (RH-3) and BSA were included as positive and negative controls, respectively.