Table 2.
Properties of LCMV-derived HLA class I-restricted CD8+ T cell epitopes
| Donor | Epitopea | Peptide sequence | ELISPOT assay result (avg no. of SFC/106 CD8+ T cells)b | Predicted HLA restriction | Measured binding affinity (nM)c | Restriction (tetramer staining)d |
|---|---|---|---|---|---|---|
| AD108 | GPC38-46 | FATCGIFAL | 63 | B*0702 | 6,230 | ND |
| GPC447-455 | YLVSIFLHL | 46 | B*1501 | 6,920 | ND | |
| NP246-254 | AAVKAGAAL | 46 | B*0702 | 9.8 | B*0702 | |
| NP414-422 | KQFKQDSKY | 73 | B*1501 | 13 | B*1501 | |
| AD123 | GPC447-455 | YLVSIFLHL | 172 | A*0201 | 35 | A*0201 |
| NP45-53 | SEVSNVQRI | 84 | B*4402 | 12 | B*4402 | |
| NP69-77 | SLNQTVHSL | 348 | A*0201 | 53 | A*0201 | |
| Z49-58 | YLCRHCLNLL | 130 | A*0201 | 3.9 | A*0201 | |
| AD124 | GPC10-18 | ALPHIIDEV | 413 | A*0201 | 57 | A*0201 |
| GPC11-19 | LPHIIDEVI | 151 | B*5401 | 1,400 | ND | |
| NP246-254 | AAVKAGAAL | 299 | B*4802 | ND | ND |
a
Peptide position within LCMV strain Armstrong clone 53b GPC, NP, or Z protein.
b
Number of net SFC per 106 purified CD8+ T cells. Data are averaged from two independent experiments.
c
Peptides were tested for binding to their predicted HLA class I allele. ND, binding was not determined because the purified HLA was not available.
d
Restriction was determined by HLA class I tetramer staining. ND, tetramer staining was not performed.