Fig. 2.

Coimmunoprecipitation of viral proteins and Snapin. Human U373MG cells were cotransfected with a combination of two plasmids expressing HA- and Myc-tagged proteins. The input protein samples (80 μg) (Input) (lanes 1, 4, 7, and 10) and samples (15 μg) that were either immunoprecipitated with anti-Myc [IP (anti-Myc)] (lanes 2, 5, 8, and 11) or anti-HA antibodies [IP (anti-HA)] (lanes 3, 6, 9, and 12) were separated on SDS-containing polyacrylamide gels and assayed with Western blot analysis using anti-Myc (A) or anti-HA (B) antibodies, respectively.