Table 1.
Plasmid constructs used in the study
| Plasmid | Description (positions [bp]) | Reference/source |
|---|---|---|
| pGBKT7 | Cloning vector for protein expression fused with GAL4 DNA-binding domain in yeast | Clontech |
| pACT2 | Cloning vector for protein expression fused with GAL4 activation domain in yeast | Clontech |
| pCMV-HA | Cloning vector for protein expression fused with hemagglutinin (HA) tag in mammalian cell | Clontech |
| pCMV-Myc | Cloning vector for protein expression fused with c-Myc tag in mammalian cell | Clontech |
| pGBKT7-UL70 | pGBKT7 containing HCMV UL70 full-length sequence (1–2841) | This study |
| pGBKT7-UL70 (1–866aa) | pGBKT7 containing UL70 C-terminally truncated sequence (1–2600) | This study |
| pGBKT7-UL70 (1–800aa) | pGBKT7 containing UL70 C-terminally truncated sequence (1–2400) | This study |
| pGBKT7-UL70 (1–733aa) | pGBKT7 containing UL70 C-terminally truncated sequence (1–2200) | This study |
| pGBKT7-UL70 (1–666aa) | pGBKT7 containing UL70 C-terminally truncated sequence (1–2000) | This study |
| pGBKT7-UL70 (1–600aa) | pGBKT7 containing UL70 C-terminally truncated sequence (1–1800) | This study |
| pGBKT7-UL70 (1–533aa) | pGBKT7 containing UL70 C-terminally truncated sequence (1–1600) | This study |
| pGBKT7-UL70 (1–466aa) | pGBKT7 containing UL70 C-terminally truncated sequence (1–1400) | This study |
| pGBKT7-UL70 (1–400aa) | pGBKT7 containing UL70 C-terminally truncated sequence (1–1200) | This study |
| pGBKT7-UL70 (67–946aa) | pGBKT7 containing UL70 N-terminally truncated sequence (199–2841) | This study |
| pGBKT7-UL70 (134–946aa) | pGBKT7 containing UL70 N-terminally truncated sequence (400–2841) | This study |
| pGBKT7-UL70 (67–733aa) | pGBKT7 containing UL70 truncated sequence (199–2200) | This study |
| pGBKT7-UL70 (67–866aa) | pGBKT7 containing UL70 truncated sequence (199–2600) | This study |
| pCMV-Myc-UL70 | pCMV-Myc containing HCMV UL70 full-length sequence (1–2841) | This study |
| pCMV-Myc-UL70 (1–866aa) | pCMV-Myc containing UL70 C-terminally truncated sequence (1–2600) | This study |
| pCMV-Myc-UL70 (1–800aa) | pCMV-Myc containing UL70 C-terminally truncated sequence (1–2400) | This study |
| pCMV-Myc-UL70 (1–733aa) | pCMV-Myc containing UL70 C-terminally truncated sequence (1–2200) | This study |
| pCMV-Myc-UL70 (1–666aa) | pCMV-Myc containing UL70 C-terminally truncated sequence (1–2000) | This study |
| pCMV-Myc-UL70 (1–600aa) | pCMV-Myc containing UL70 C-terminally truncated sequence (1–1800) | This study |
| pCMV-Myc-UL70 (1–533aa) | pCMV-Myc containing UL70 C-terminally truncated sequence (1–1600) | This study |
| pCMV-Myc-UL70 (1–466aa) | pCMV-Myc containing UL70 C-terminally truncated sequence (1–1400) | This study |
| pCMV-Myc-UL70 (1–400aa) | pCMV-Myc containing UL70 C-terminally truncated sequence (1–1200) | This study |
| pCMV-Myc-UL70 (67–946aa) | pCMV-Myc containing UL70 N-terminally truncated sequence (199–2841) | This study |
| pCMV-Myc-UL70 (134–946aa) | pCMV-Myc containing UL70 N-terminally truncated sequence (400–2841) | This study |
| pCMV-Myc-UL70 (67–733aa) | pCMV-Myc containing UL70 truncated sequence (199–2200) | This study |
| pCMV-Myc-UL70 (67–866aa) | pCMV-Myc containing UL70 truncated sequence (199–2600) | This study |
| pCMV-Snapin | pCMV containing full-length human Snapin-encoding sequence (1–411) | This study |
| pCMV-HA-Snapin | pCMV-HA containing full-length human Snapin-encoding sequence (1–411) | This study |