Table 1.
Replication of UL15 mutants on various cell lines
| Virus | Genotype of UL15a | Cell lines usedb | Mean titer ± SD (PFU/ml)c |
|---|---|---|---|
| vJB27 | Del 400-420aa | CV1/CV15 | <102 |
| vJB27 | CV15/CV15 | (3.2 ± 0.76) × 106 | |
| vJB28 | Del 383-385aa | CV1/CV15 | <102 |
| vJB28 | CV15/CV15 | (2 ± 0.45) × 106 | |
| vJB27R | Restored 400-420aa deletion | CV1/CV1 | (3.3 ± 1.5) × 107 |
| vJB28R | Restored 383-385aa deletion | CV1/CV1 | (2.9 ± 0.96) × 107 |
| HSV-1(F) | Wild type | CV1/CV1 | (2.5 ± 1.32) × 107 |
a
aa, amino acids.
b
Expressed as the used cells for testing virus replication/the cells used for virus quantification.
c
Virus titers were determined in triplicate by plaque assay as described in Materials and Methods. The data represent means for three independent experiments.