Fig. 7.

Interaction of wild-type and mutant pUL34 and pUL31 in transfected cells. (A to F) Digital confocal images of Vero cells transfected with pUL31-FLAG and pUL34 expression constructs are shown. At 48 h after transfection, cells were fixed and immunofluorescently stained for pUL34 using anti-UL34 antibody (green) or for pUL31-FLAG using anti-FLAG antibody (red). The constructs trans- fected for each image are indicated to the left of the panel. (G) Quantitation of pUL31 colocalization with pUL34. Under each transfection condition, 50 randomly chosen cells were scored for the amount of pUL31 colocalized with pUL34. Note that while pUL31 might be completely colocalized with pUL34, the reverse was rarely observed (i.e., there was almost always pUL34 that was not colocalized with pUL31).