Fig. 5.

Intracellular distribution of viral proteins in the presence of MG132. MA104 cells were infected with RRV at an MOI of 1 and incubated with MEM plus NEAA in the presence (B, D, F, H, b, d, f, and h) or absence (A, C, E, G, a, c, e, and g) of 10 μM MG132. At 8 hpi, the cells were fixed and immunostained as indicated in Materials and Methods with antibodies to VP1 (A and B), VP2 (C and D), VP6 (E and F), or NSP2 (G and H). The cells in panels a to h were all stained with antibodies to NSP5.