Fig. 6.

The E1 ubiquitin-activating enzyme has a role in rotavirus replication. Murine fibroblasts ts20b and H38.5 were infected with RRV at an MOI of 1 at the permissive temperature (32°C) for 1 h. The cells were subsequently incubated at either 32°C or 39°C for 8 h, and the yield of infectious viral progeny was determined by an immunoperoxidase assay. The results represent the arithmetic means ± SEM of 3 independent experiments performed in duplicate. **, P < 0.01.