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Agarose gel electrophoresis of total DNA isolated from virus-infected BGE-2 cells at different times p.i. (3 h, 1 day; and 5 days). V1 to V13 are the numbers of passages through BGE-2 cells, with approximately one passage per 10 to 14 days. The regular arrow marks the total genomic DNA, the single-feathered arrow marks BgDNV genomic DNA ∼5 kb in size, and the double-feathered arrow marks the replicative form of BgDNV DNA. The DNA size markers on the left are in kilobases.
