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. 2011 Nov;85(22):11638–11645. doi: 10.1128/JVI.05659-11

Fig. 1.

Fig. 1.

Interference mediated by HCMV glycoproteins gH/gL/gO and gH/gL/UL128-131 in epithelial cells and fibroblasts. ARPE-19 epithelial cells (A), normal human foreskin fibroblasts (HFFs) (B), or MRC-5 fibroblasts (C) were transduced for 24 h with the indicated combinations of Ad vectors expressing HCMV glycoproteins, as previously described (26). The cells were then infected with HCMV strain TR (2 PFU per cell) for an additional 48 h and stained for the HCMV IE-86 protein as described previously (26). Under each condition, at least 350 cells were characterized for IE-86 expression in each of three random fields. Relative HCMV infection refers to the average number of IE-86-positive cells normalized to the average number of infected cells observed in monolayers infected with a control Ad vector expressing GFP (set at 1.0). HCMV infected 50 to 60% of ARPE-19 cells and 90 to 100% of the HFFs and MRC-5 cells transduced with Ad-GFP. Error bars indicate the standard deviations from the means. Results shown are representative of three separate experiments.

HHS Vulnerability Disclosure