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. 2011 Nov;85(22):11601–11614. doi: 10.1128/JVI.05239-11

Fig. 7.

Fig. 7.

Effects of HIV-1 proteins and/or morphine on HCV NS3 and core protein levels in HCV JFH1-infected Huh7.5.1 cells at 8 h following exposure. HCV (JFH1)-infected Huh7.5.1 cells were pretreated with the proteasome inhibitor MG132 (10 μM) or the ROS inhibitor N-acetyl cysteine (NAC) (10 μM), followed by incubation with HIV-1 Tat (100 nM) or bitropic gp120MN (500 pM) with or without morphine (M) (500 nM) and assessed at 8 h following treatment. (A) HCV NS3 protein was assayed by Western blot analysis and normalized to β-actin. Data are mean change in NS3 protein levels relative to HCV infection alone (percentage of control) ± SEM from three independent experiments (*, P < 0.05 versus control; a, P < 0.05 versus HIV-1 alone; #, P < 0.05 versus HCV JFH1 without inhibitor). (B) HCV core levels were assayed by ELISA. Values are mean HCV core protein levels (pg/ml) ± SEM from three independent experiments (*, P < 0.05 versus untreated, HCV-infected controls; a, P < 0.05 versus HIV-1 protein alone; b, P < 0.05 versus morphine alone; #, P < 0.05 versus HCV-infected controls without inhibitor).