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. 2011 Nov;85(22):11685–11696. doi: 10.1128/JVI.05726-11

Fig. 5.

Fig. 5.

Inverse PCR clones confirm that MdBV segments J and C integrate into the genome of CiE1 cells. (A) Schematic illustrating the two left (CiJ1L and CiJ2L) and one right (CiJ1R) segment J-CiE1 junction sequences cloned by inverse PCR. Each junction clone is aligned with segment J linearized at nt 3262 (left) and nt 3211 (right). The MdBV sequence in each junction clone is highlighted in black, and the CiE1 genomic sequence is highlighted in white. Note that the segment J boundary for both left clones corresponds to nt 3262 and the tetramer ACCA, while the boundary for the right junction clone corresponds to nt 3211 and the tetramer TAGT. The analyzed CiE1 sequences for the two left junction clones are 794 and 39 bp long, respectively, while the CiE1 sequence for the right junction clone is 931 bp. (B) Schematic illustrating the one right (CiC1R) segment C-CiE1 junction sequence cloned by inverse PCR. The schematic is organized as described above (A). The CiE1 sequence for the clone is 990 bp.