Fig. 6.

PCR-based integration assays and inverse PCR clones confirm that MdBV segments integrate into the genome of parasitized P. includens. (A) PCR products amplified using domain-specific primers for segments B, C, and J and hemocyte genomic DNA collected from parasitized P. includens larvae (day 7 postparasitism). Domains correspond to the domains shown in Fig. 3. (B) Schematic illustrating the one left (PiJ1L) and one right (PiJ1R) segment J-P. includens junction sequences cloned by inverse PCR. The schematic is organized as described in the legend of Fig. 5. The P. includens (Pi) sequence for the left junction clone is 339 bp long, while the P. includens sequence for the right junction clone is 737 bp. (C) Schematic illustrating the one left (PiC1L) and one right (PiC1R) segment C-P. includens junction sequences cloned by inverse PCR. The P. includens sequence for the left junction clone is 473 bp long, while the P. includens sequence for the right junction clone is 68 bp.