Table 3.
Detection of JV by ELISA and RT-PCR in fecal swabs or intestinal contents of calvesa
| Necropsy time | Calf no. | FS/IC | Time(s) of sampling (hpi) | Result |
|
|---|---|---|---|---|---|
| ELISA | RT-PCR | ||||
| 12 hpi | JV-1 | FS | 6.0, 12.0 | 0 | 0 |
| IC | 12.0 | 0 | + | ||
| JV-2 | FS | 6.0 | 0 | 0 | |
| IC | 12.0 | + | 0 | ||
| Co-1 | FS | NT | NT | NT | |
| IC | 12.5 | 0 | 0 | ||
| Co-2 | FS | 7.0 | 0 | 0 | |
| IC | 13.0 | 0 | 0 | ||
| 18-21 hpi | JV-3 | FS | 6.0 | 0 | 0 |
| FS | 13.0 | + | + | ||
| FS | 15.0 | + | 0 | ||
| IC | NT | NT | NT | ||
| JV-4 | FS | 5.0 | 0 | 0 | |
| FS | 16.0 | 0 | + | ||
| FS | 17.0, 17.5, 18.5 | + | + | ||
| IC | 19,0 | + | 0 | ||
| Co-3 | FS | 7.0 | 0 | 0 | |
| Co-4 | IC | 20,5 | 0 | 0 | |
| 4 dpi | JV-5 | FS | 9.0 | 0 | 0 |
| FS | 14.0, 19.0, 21.0, 23.0 | + | 0 | ||
| FS | 25.0, 27.0, 33.0, 43.0, 47.0, 49.0, 54.0, 57.0, 67.0, 79.0 | 0 | 0 | ||
| IC | 90.0 | 0 | 0 | ||
| JV-6 | FS | 12.0 | 0 | + | |
| FS | 14.0, 16.0, 18.0, 20.0 | + | + | ||
| FS | 32.0, 46.0, 50.0, 56.0, 68.0, 80.0 | 0 | + | ||
| IC | 92.0 | 0 | + | ||
| Co-5 | FS | 9.5, 19.5, 43.5, 92.0 | 0 | 0 | |
| IC | 92.5 | 0 | 0 | ||
| Co-6 | FS | 19.5, 43.5, 67.5, 91.5 | 0 | 0 | |
| IC | 95.5 | 0 | 0 | ||
FS, fecal swabs; IC, intestinal contents; NT, not tested; +, JV positive; 0, JV negative.