Fig. 2.

miR-US5-1 and miR-US5-2 act synergistically to downregulate US7. (A) Luciferase assays were performed on cells cotransfected with the US7 WT 3′UTR luciferase reporter construct and various combinations of miR-US5-1 and miR-US5-2 as indicated in Materials and Methods. Dose pairs of miRNAs yielding 50% inhibition of luciferase activity were determined from titration curves and plotted onto a Cartesian graph. The line connecting the plots corresponding to miR-US5-1 and miR-US5-2 alone is the line of additivity. Dots located below the line of additivity indicate synergy. (B) Analysis of cells transfected with the US7 WT 3′UTR luciferase reporter construct and dilutions of miR-US5-2.