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miR-US5-1 interacts with the US7 3′UTR at one functional site. Site-directed mutagenesis was used to mutate the predicted miR-US5-1 target site in the US7 3′UTR luciferase reporter construct. Reporter constructs encoding the wild-type US7 3′UTR or the mutated US7 3′UTR (US5-1 Mutant) were cotransfected into cells with either a negative control miRNA (NEG) or the indicated miR(s). Dual luciferase analyses were performed to examine reporter activity 16 h posttransfection.
