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. 2011 Nov;85(22):11891–11900. doi: 10.1128/JVI.00832-11

Fig. 1.

Fig. 1.

Effect of PTEN/PI3K/PDK1/AKT signal transduction pathway on HBV biosynthesis in the human hepatoma cell line HepG2. (A and B) Cells were transfected with the HBV DNA (4.1-kbp) construct alone (lane 1) or the HBV DNA (4.1-kbp) construct plus the PGC1α and PTEN expression vectors (lanes 2 to 9), as indicated. (C and D) Cells were treated with the PI3K inhibitor LY294002 (10 μM), as indicated. (A and C) RNA (Northern) filter hybridization analysis of HBV transcripts. The glyceraldehyde-3-phosphate dehydrogenase (GAPDH) transcript was used as an internal control for RNA loading per lane. (B and D) DNA (Southern) filter hybridization analysis of HBV replication intermediates. HBV RC DNA, HBV relaxed circular DNA; HBV SS DNA, HBV single-stranded DNA.